Hotspot

ycf66-psbM

Genome · 91762-91961 · 200 bp

Back to Sphagnum

Pi 0.0272

MarkerSeek score 74.80

Primer available yes

Label ycf66-psbM

Feature Metadata

label_name: ycf66-psbM
feature_type: igs
region: Genome
length_bp: 200
variable_sites: 15
pi: 0.0272
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66-psbM_p1	AAGTAGGCCTACCACGATGA	TGCTACTGCACTGTTCATACT	50.0	42.9	57.8	57.0	333-349 bp	1.0	70.6
ycf66-psbM_p2	AAGTAGGCCTACCACGATGA	CGTACAAACAGTCAGTCAAGG	50.0	47.6	57.8	56.9	282-298 bp	1.0	69.9
ycf66-psbM_p3	AGTAGGCCTACCACGATGAG	TGCTACTGCACTGTTCATACT	55.0	42.9	58.3	57.0	332-348 bp	1.0	69.4
ycf66-psbM_p4	AGTAGGCCTACCACGATGAG	CGTACAAACAGTCAGTCAAGG	55.0	47.6	58.3	56.9	281-297 bp	1.0	68.7
ycf66-psbM_p5	AAGTAGGCCTACCACGATGA	TGCTACTGCACTGTTCATAC	50.0	45.0	57.8	55.5	333-349 bp	1.0	66.2