Hotspot

atpF-atpH

Genome · 76653-77002 · 350 bp

Back to Sphagnum

Pi 0.0153

MarkerSeek score 63.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 350
variable_sites: 15
pi: 0.0153
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AATTTCCAGCAAGAGGCCAA	AGTCGTAGCTCTAGCACCTT	45.0	50.0	58.0	57.9	430-449 bp	1.0	84.9
atpF-atpH_p2	AAATTTCCAGCAAGAGGCCA	AGTCGTAGCTCTAGCACCTT	45.0	50.0	58.0	57.9	431-450 bp	1.0	84.8
atpF-atpH_p3	ATTTCCAGCAAGAGGCCAAT	AGTCGTAGCTCTAGCACCTT	45.0	50.0	57.8	57.9	429-448 bp	1.0	83.0
atpF-atpH_p4	GCCAATACCCTGGAGGAATC	AGTCGTAGCTCTAGCACCTT	55.0	50.0	57.7	57.9	415-434 bp	1.0	82.9
atpF-atpH_p5	TTCCAGCAAGAGGCCAATAC	AGTCGTAGCTCTAGCACCTT	50.0	50.0	57.9	57.9	427-446 bp	0.9	79.1