Hotspot

trnK-UUU-chlB

Genome · 67024-68381 · 1358 bp

Back to Sphagnum

Pi 0.0229

MarkerSeek score 63.20

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 1358
variable_sites: 86
pi: 0.0229
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	AGTCGCACTTAAAAGCCGAG	ACGACAAAATGGCATTGCAG	50.0	45.0	58.6	57.9	1454-1488 bp	1.0	83.1
trnK-UUU-chlB_p2	AGTCGCACTTAAAAGCCGAG	CACGACAAAATGGCATTGCA	50.0	45.0	58.6	58.2	1455-1489 bp	1.0	81.8
trnK-UUU-chlB_p3	AGTCGCACTTAAAAGCCGAG	TGCACGACAAAATGGCATTG	50.0	45.0	58.6	58.2	1457-1491 bp	1.0	81.8
trnK-UUU-chlB_p4	TCGCACTTAAAAGCCGAGTA	ACGACAAAATGGCATTGCAG	45.0	45.0	57.3	57.9	1452-1486 bp	1.0	81.8
trnK-UUU-chlB_p5	CGCACTTAAAAGCCGAGTAC	ACGACAAAATGGCATTGCAG	50.0	45.0	57.2	57.9	1451-1485 bp	1.0	81.4