Hotspot

trnT-GGU-trnE-UUC

Genome · 53354-54206 · 853 bp

Back to Sphagnum

Pi 0.0167

MarkerSeek score 62.80

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: Genome
length_bp: 853
variable_sites: 41
pi: 0.0167
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.0	950-1959 bp	1.0	83.0
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	GTCGCCTCCTTGAAAGAGAG	55.0	55.0	58.1	58.0	929-1938 bp	1.0	82.6
trnT-GGU-trnE-UUC_p3	TGGCGTTACTCTACCACTGA	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	58.1	58.0	926-1935 bp	1.0	82.4
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	GCCTCCTTGAAAGAGAGGTG	50.0	55.0	58.0	57.9	947-1956 bp	1.0	82.2
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	GGTTTCTTCATGGTACCCCC	50.0	55.0	58.0	57.9	988-1997 bp	1.0	81.8