Hotspot

ndhJ-trnF-GAA

Genome · 37198-37645 · 448 bp

Back to Sphagnum

Pi 0.0218

MarkerSeek score 59.40

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: Genome
length_bp: 448
variable_sites: 25
pi: 0.0218
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	GCCCGATAGTTGGATTGGTT	ATTCGAATGACAGAAGCCGG	50.0	50.0	57.9	58.3	611-622 bp	1.0	80.6
ndhJ-trnF-GAA_p2	GTTGGATTGGTTGGCCCTTA	ATTCGAATGACAGAAGCCGG	50.0	50.0	58.1	58.3	603-614 bp	1.0	80.5
ndhJ-trnF-GAA_p3	TTGGATTGGTTGGCCCTTAC	ATTCGAATGACAGAAGCCGG	50.0	50.0	58.1	58.3	602-613 bp	1.0	80.5
ndhJ-trnF-GAA_p4	ATAGTTGGATTGGTTGGCCC	ATTCGAATGACAGAAGCCGG	50.0	50.0	57.8	58.3	606-617 bp	1.0	79.8
ndhJ-trnF-GAA_p5	TGGTTGGCCCTTACGTAAAG	ATTCGAATGACAGAAGCCGG	50.0	50.0	57.8	58.3	596-607 bp	1.0	79.6