Hotspot

trnV-UAC-ndhC

Genome · 34605-35528 · 924 bp

Back to Sphagnum

Pi 0.0202

MarkerSeek score 66.70

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 924
variable_sites: 55
pi: 0.0202
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GGTTCGAATCCGTATAGCCC	ATCATATTTGGCCAGTGGGG	55.0	50.0	57.9	57.6	968-992 bp	1.0	83.6
trnV-UAC-ndhC_p2	TGCATGTTGGGTTCCTGAAA	ATCATATTTGGCCAGTGGGG	45.0	50.0	57.9	57.6	1062-1087 bp	1.0	83.0
trnV-UAC-ndhC_p3	CCGAGAATGTCTACGGTTCG	ATCATATTTGGCCAGTGGGG	55.0	50.0	58.2	57.6	982-1006 bp	1.0	82.7
trnV-UAC-ndhC_p4	CGGTTCGAATCCGTATAGCC	ATCATATTTGGCCAGTGGGG	55.0	50.0	58.3	57.6	969-993 bp	1.0	81.8
trnV-UAC-ndhC_p5	GGGTTCCTGAAACGGTTCAA	ATCATATTTGGCCAGTGGGG	50.0	50.0	58.3	57.6	1054-1079 bp	1.0	81.1