Hotspot

psbJ-petA

Genome · 22856-23886 · 1031 bp

Back to Sphagnum

Pi 0.0238

MarkerSeek score 64.30

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 1031
variable_sites: 68
pi: 0.0238
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	AGGTACTGTAGCTGGTATCCT	TCGAAAAAGTTCAATTAGCTGAGA	47.6	33.3	57.0	56.9	1144-1169 bp	1.0	46.0
psbJ-petA_p2	AGGTACTGTAGCTGGTATCCTT	TCGAAAAAGTTCAATTAGCTGAGA	45.5	33.3	57.7	56.9	1144-1169 bp	1.0	46.0
psbJ-petA_p3	GGTACTGTAGCTGGTATCCTTG	TCGAAAAAGTTCAATTAGCTGAGA	50.0	33.3	57.7	56.9	1143-1168 bp	1.0	46.0
psbJ-petA_p4	AGGTACTGTAGCTGGTATCCT	CGAAAAAGTTCAATTAGCTGAGA	47.6	34.8	57.0	55.5	1143-1168 bp	1.0	46.0
psbJ-petA_p5	AGGTACTGTAGCTGGTATCCT	TCGAAAAAGTTCAATTAGCTGAG	47.6	34.8	57.0	55.5	1144-1169 bp	1.0	46.0