Hotspot

psaJ-trnP-UGG

Genome · 18854-19507 · 654 bp

Back to Sphagnum

Pi 0.019

MarkerSeek score 61.60

Primer available yes

Label psaJ-trnP-UGG

Feature Metadata

label_name: psaJ-trnP-UGG
feature_type: igs
region: Genome
length_bp: 654
variable_sites: 37
pi: 0.019
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP-UGG_p1	GGCGCCGTAGAAAGATATGT	GGGATGACAGGATTCGAACC	50.0	55.0	57.8	58.0	761-775 bp	1.0	83.6
psaJ-trnP-UGG_p2	GGCGCCGTAGAAAGATATGT	AAAACAAACGCGCTACCAAG	50.0	45.0	57.8	57.9	724-738 bp	1.0	83.3
psaJ-trnP-UGG_p3	GGCGCCGTAGAAAGATATGT	CAGGGTAGGGATGACAGGAT	50.0	55.0	57.8	57.9	768-782 bp	1.0	83.0
psaJ-trnP-UGG_p4	GGCGCCGTAGAAAGATATGT	TTCAGGGTAGGGATGACAGG	50.0	55.0	57.8	58.1	770-784 bp	1.0	82.9
psaJ-trnP-UGG_p5	TAAGCCAGCCAAAAATCCGA	GGGATGACAGGATTCGAACC	45.0	55.0	57.8	58.0	801-815 bp	0.9	78.7