Hotspot

clpP-rps12

Genome · 16031-16332 · 302 bp

Back to Sphagnum

Pi 0.0203

MarkerSeek score 71.00

Primer available yes

Label clpP-rps12

Feature Metadata

label_name: clpP-rps12
feature_type: igs
region: Genome
length_bp: 302
variable_sites: 21
pi: 0.0203
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rps12_p1	TGTCGGCAAAAGAAGCAAAA	CGGTTGTCTTCGATTCCTGA	40.0	50.0	57.1	57.6	389-415 bp	1.0	77.8
clpP-rps12_p2	TGTCGGCAAAAGAAGCAAAA	CCCGAAGAGCTGGAGATTTT	40.0	50.0	57.1	57.6	423-449 bp	1.0	77.3
clpP-rps12_p3	AGAGACGTTTTTATGTCGGCA	CGGTTGTCTTCGATTCCTGA	42.9	50.0	58.0	57.6	402-428 bp	1.0	77.0
clpP-rps12_p4	AGAGACGTTTTTATGTCGGCA	CCCGAAGAGCTGGAGATTTT	42.9	50.0	58.0	57.6	436-462 bp	1.0	76.5
clpP-rps12_p5	ATGTCGGCAAAAGAAGCAAA	CGGTTGTCTTCGATTCCTGA	40.0	50.0	56.8	57.6	390-416 bp	1.0	76.0