Hotspot

rpl16-rpl14

Genome · 4956-5124 · 169 bp

Back to Sphagnum

Pi 0.0358

MarkerSeek score 62.70

Primer available yes

Label rpl16-rpl14

Feature Metadata

label_name: rpl16-rpl14
feature_type: igs
region: Genome
length_bp: 169
variable_sites: 14
pi: 0.0358
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rpl14_p1	CCGATAAAAATATGAGTGATGGGT	CAGTTTTCGAGCCCCACTAT	37.5	50.0	56.9	57.6	243-252 bp	1.0	48.8
rpl16-rpl14_p2	TCCGATAAAAATATGAGTGATGGGT	CAGTTTTCGAGCCCCACTAT	36.0	50.0	58.2	57.6	244-253 bp	1.0	48.8
rpl16-rpl14_p3	CCGATAAAAATATGAGTGATGGGT	CGAGCCCCACTATTATCTGC	37.5	55.0	56.9	57.9	236-245 bp	1.0	48.6
rpl16-rpl14_p4	TCCGATAAAAATATGAGTGATGGGT	CGAGCCCCACTATTATCTGC	36.0	55.0	58.2	57.9	237-246 bp	1.0	48.6
rpl16-rpl14_p5	TCCGATAAAAATATGAGTGATGGG	CGAGCCCCACTATTATCTGC	37.5	55.0	56.6	57.9	237-246 bp	1.0	48.6