Hotspot

trnI-CAU-rpl23

Genome · 174-417 · 244 bp

Back to Sphagnum

Pi 0.0183

MarkerSeek score 44.80

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: Genome
length_bp: 244
variable_sites: 15
pi: 0.0183
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	GGTTCAACTCCTGTCGGATG	ACCGGGTATTTCATTTCATCCA	55.0	40.9	58.3	57.8	288-304 bp	1.0	64.3
trnI-CAU-rpl23_p2	AGGTTCAACTCCTGTCGGAT	ACCGGGTATTTCATTTCATCCA	50.0	40.9	58.4	57.8	289-305 bp	1.0	63.6
trnI-CAU-rpl23_p3	CGGTTAAAGCACCCAACTCA	ACCGGGTATTTCATTTCATCCA	50.0	40.9	58.4	57.8	325-341 bp	1.0	62.7
trnI-CAU-rpl23_p4	TGGAGAATTCGCAGGTTCAA	ACCGGGTATTTCATTTCATCCA	45.0	40.9	57.4	57.8	301-317 bp	1.0	61.8
trnI-CAU-rpl23_p5	TTGGAGAATTCGCAGGTTCA	ACCGGGTATTTCATTTCATCCA	45.0	40.9	57.4	57.8	302-318 bp	1.0	61.8