Hotspot

rps15-trnN-GUU

SSC · 121818-127592 · 5775 bp

Back to Sphagneticola

Pi 0.0166
MarkerSeek score 49.70
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
5775
variable_sites
95
pi
0.0166
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 TGACACTCCCCTTTTTACAGA AATTATGGAACCTGCCCGTC 42.9 50.0 56.5 57.9 884-902 bp 1.0 61.5
rps15-trnN-GUU_p2 TGACACTCCCCTTTTTACAGA TATGGAACCTGCCCGTCTAT 42.9 50.0 56.5 57.9 881-899 bp 1.0 61.1
rps15-trnN-GUU_p3 TGACACTCCCCTTTTTACAGA TTATGGAACCTGCCCGTCTA 42.9 50.0 56.5 58.1 882-900 bp 1.0 60.9
rps15-trnN-GUU_p4 TGACACTCCCCTTTTTACAGA AACCTGCCCGTCTATCTGTA 42.9 50.0 56.5 57.8 876-894 bp 1.0 60.7
rps15-trnN-GUU_p5 TGACACTCCCCTTTTTACAGA ACCTGCCCGTCTATCTGTAA 42.9 50.0 56.5 57.8 875-893 bp 1.0 60.7