Hotspot

ndhI-ndhA

SSC · 118068-119697 · 1630 bp

Back to Sphagneticola

Pi 0.0167

MarkerSeek score 57.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 1630
variable_sites: 27
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	GCTCGTATTGTTTGTTGGCC	TTAGGTGGTCTACGAGCTGC	50.0	55.0	58.0	59.2	1776-1802 bp	1.0	72.0
ndhI-ndhA_p2	GGCTCGTATTGTTTGTTGGC	TTAGGTGGTCTACGAGCTGC	50.0	55.0	58.0	59.2	1777-1803 bp	1.0	72.0
ndhI-ndhA_p3	GCTCGTATTGTTTGTTGGCC	TTTAGGTGGTCTACGAGCTG	50.0	50.0	58.0	56.7	1777-1803 bp	1.0	71.0
ndhI-ndhA_p4	GGCTCGTATTGTTTGTTGGC	TTTAGGTGGTCTACGAGCTG	50.0	50.0	58.0	56.7	1778-1804 bp	1.0	71.0
ndhI-ndhA_p5	CGGCTCGTATTGTTTGTTGG	TTAGGTGGTCTACGAGCTGC	50.0	55.0	57.8	59.2	1778-1804 bp	1.0	70.3