Hotspot

rpl32-trnL-UAG

SSC · 111818-112587 · 770 bp

Back to Sphagneticola

Pi 0.0418
MarkerSeek score 76.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
770
variable_sites
31
pi
0.0418
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCTCTTTCTACCGGG AGCGTGTCTACCGATTTCAC 52.4 50.0 57.8 58.0 862-888 bp 1.0 75.9
rpl32-trnL-UAG_p2 GGGAAATCTCTTTCTACCGGG CAGCGTGTCTACCGATTTCA 52.4 50.0 57.8 58.0 863-889 bp 1.0 75.8
rpl32-trnL-UAG_p3 GGGAAATCTCTTTCTACCGGG TCCTAAGAGCAGCGTGTCTA 52.4 50.0 57.8 57.9 872-898 bp 1.0 74.9
rpl32-trnL-UAG_p4 GGGAAATCTCTTTCTACCGGG CCTAAGAGCAGCGTGTCTAC 52.4 55.0 57.8 57.8 871-897 bp 1.0 74.3
rpl32-trnL-UAG_p5 GGGAAATCTCTTTCTACCGGG CTAAGAGCAGCGTGTCTACC 52.4 55.0 57.8 57.8 870-896 bp 1.0 74.3