Hotspot

ndhF-rpl32

SSC · 110646-111652 · 1007 bp

Back to Sphagneticola

Pi 0.0357

MarkerSeek score 72.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1007
variable_sites: 35
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TCCAAATATTTTTACGAATACGCT	50.0	29.2	58.0	55.3	1150-1166 bp	1.0	44.3
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TCCAAATATTTTTACGAATACGCT	45.0	29.2	58.0	55.3	1148-1164 bp	1.0	44.3
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TCCAAATATTTTTACGAATACGCT	45.0	29.2	58.0	55.3	1149-1165 bp	1.0	44.3
ndhF-rpl32_p4	CTTTTTGTTGCCGTCGGAAA	TCCAAATATTTTTACGAATACGCT	45.0	29.2	57.8	55.3	1156-1172 bp	1.0	44.3
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TCCAAATATTTTTACGAATACGCT	45.0	29.2	57.8	55.3	1153-1169 bp	1.0	44.3