Hotspot

rpl16-rps3

LSC · 80753-81905 · 1153 bp

Back to Sphagneticola

Pi 0.0221

MarkerSeek score 64.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 25
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GGAATCTGGTTCTTTTGGGGT	CCACTACAAACCATTCGAGC	47.6	50.0	58.1	56.8	1289-1319 bp	1.0	64.5
rpl16-rps3_p2	GGAATCTGGTTCTTTTGGGGT	CCACTACAAACCATTCGAGCT	47.6	47.6	58.1	58.3	1289-1319 bp	1.0	63.6
rpl16-rps3_p3	GGAATCTGGTTCTTTTGGGGT	TCCACTACAAACCATTCGAGC	47.6	47.6	58.1	58.3	1290-1320 bp	1.0	63.6
rpl16-rps3_p4	CGGAATCTGGTTCTTTTGGG	CCACTACAAACCATTCGAGC	50.0	50.0	56.7	56.8	1290-1320 bp	1.0	63.0
rpl16-rps3_p5	CGGAATCTGGTTCTTTTGGG	CCACTACAAACCATTCGAGCT	50.0	47.6	56.7	58.3	1290-1320 bp	1.0	62.1