Hotspot

infA-rps8

LSC · 79154-79265 · 112 bp

Back to Sphagneticola

Pi 0.0194

MarkerSeek score 67.50

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 2
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CGTTGGGAAGCGATTCAGTA	CGGGATGGGGGTTGTAATTC	50.0	55.0	58.0	58.3	270 bp	1.0	80.7
infA-rps8_p2	CGTTGGGAAGCGATTCAGTA	AGAATTTTAGGCGGGATGGG	50.0	50.0	58.0	57.6	281 bp	1.0	80.2
infA-rps8_p3	CGTTGGGAAGCGATTCAGTA	CGGGGTATAATGACAGACCG	50.0	55.0	58.0	57.6	239 bp	1.0	80.2
infA-rps8_p4	CGTTGGGAAGCGATTCAGTA	TTCTTTCTACCTCTCGGGGT	50.0	50.0	58.0	57.4	253 bp	1.0	78.5
infA-rps8_p5	CGTTGGGAAGCGATTCAGTA	GAATTTTAGGCGGGATGGGG	50.0	55.0	58.0	58.7	280 bp	1.0	77.7