Hotspot

accD-psaI

LSC · 57642-58414 · 773 bp

Back to Sphagneticola

Pi 0.0346

MarkerSeek score 72.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 25
pi: 0.0346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAACTCCACACTTTCTTTCCT	AAAGAAGCCATTGCGATTGC	40.9	45.0	57.5	58.0	859-887 bp	1.0	66.3
accD-psaI_p2	TCAACTCCACACTTTCTTTCCT	TTGCGATTGCCGGAAATACT	40.9	45.0	57.5	58.3	849-877 bp	1.0	64.2
accD-psaI_p3	TCAACTCCACACTTTCTTTCCT	AGGCCTACTAAAGGGACCAA	40.9	50.0	57.5	57.6	829-857 bp	1.0	63.5
accD-psaI_p4	TCAACTCCACACTTTCTTTCCT	GGGACCAAAACAGAGGGAAA	40.9	50.0	57.5	57.6	817-845 bp	1.0	63.4
accD-psaI_p5	TCAACTCCACACTTTCTTTCCT	AAAGGGACCAAAACAGAGGG	40.9	50.0	57.5	57.6	820-848 bp	1.0	63.3