Hotspot

trnG-UCC-trnfM-CAU

LSC · 36033-36224 · 192 bp

Back to Sphagneticola

Pi 0.033

MarkerSeek score 76.00

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 6
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	260-270 bp	1.0	83.2
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	296-306 bp	1.0	82.7
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	254-264 bp	1.0	82.7
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	253-263 bp	1.0	82.2
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TTCAGTTCTTCTCTTCGGCG	50.0	50.0	57.8	57.9	318-328 bp	1.0	82.1