Hotspot

psbI-trnS-GCU

LSC · 8409-8547 · 139 bp

Back to Sphagneticola

Pi 0.037

MarkerSeek score 72.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 4
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	223-255 bp	1.0	77.0
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	171-203 bp	1.0	75.1
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	223-255 bp	1.0	74.7
psbI-trnS-GCU_p4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	242-274 bp	1.0	73.8
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	214-246 bp	1.0	72.8