Hotspot

ycf66

Genome · 29120-29822 · 703 bp

Back to Sphaeropteris

Pi 0.0259

MarkerSeek score 60.80

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 703
variable_sites: 18
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	TTCGTAGCCACTGCACTTTT	CTGCAGTTTGGGGAAGAAGT	45.0	50.0	58.0	58.0	1303-1307 bp	1.0	81.4
ycf66_p2	AGGTTGCAACAGCTGATTGA	CTGCAGTTTGGGGAAGAAGT	45.0	50.0	58.0	58.0	1608-1612 bp	1.0	81.2
ycf66_p3	TGAGGTTGCAACAGCTGATT	CTGCAGTTTGGGGAAGAAGT	45.0	50.0	58.0	58.0	1610-1614 bp	1.0	81.2
ycf66_p4	AGGTTGCAACAGCTGATTGA	AGACGGAGGATTGCAAATCC	45.0	50.0	58.0	58.0	2122-2124 bp	1.0	80.8
ycf66_p5	TGAGGTTGCAACAGCTGATT	AGACGGAGGATTGCAAATCC	45.0	50.0	58.0	58.0	2124-2126 bp	1.0	80.8