Hotspot

trnR-ACG-trnT-UGU

Genome · 153758-154855 · 1098 bp

Back to Sphaeropteris

Pi 0.00924

MarkerSeek score 65.50

Primer available yes

Label trnR-ACG-trnT-UGU

Feature Metadata

label_name: trnR-ACG-trnT-UGU
feature_type: igs
region: Genome
length_bp: 1098
variable_sites: 10
pi: 0.00924
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnT-UGU_p1	AAAAGTGGTTCCTGGAACGG	ACGTTCTCCTGCCTCGATAT	50.0	50.0	58.3	58.3	1293-1309 bp	1.0	76.6
trnR-ACG-trnT-UGU_p2	AAAAGTGGTTCCTGGAACGG	TACGTTCTCCTGCCTCGATA	50.0	50.0	58.3	57.7	1294-1310 bp	1.0	76.4
trnR-ACG-trnT-UGU_p3	AAAAGTGGTTCCTGGAACGG	TACTACGTTCTCCTGCCTCG	50.0	55.0	58.3	58.6	1297-1313 bp	1.0	74.1
trnR-ACG-trnT-UGU_p4	AAAAGTGGTTCCTGGAACGG	TCTACTACGTTCTCCTGCCT	50.0	50.0	58.3	57.2	1299-1315 bp	1.0	72.7
trnR-ACG-trnT-UGU_p5	AAGTGGTTCCTGGAACGGAT	ACGTTCTCCTGCCTCGATAT	50.0	50.0	58.9	58.3	1291-1307 bp	1.0	71.7