Hotspot

rps15-ycf1

Genome · 123736-124869 · 1134 bp

Back to Sphaeropteris

Pi 0.0194

MarkerSeek score 68.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 1134
variable_sites: 22
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGCAGATCCTGTGCCTTTTT	CAGCCACCGTCTAGAAGAAC	45.0	55.0	57.7	58.0	1308-1332 bp	1.0	79.0
rps15-ycf1_p2	AGCAGATCCTGTGCCTTTTT	AGCCACCGTCTAGAAGAACT	45.0	50.0	57.7	58.1	1307-1331 bp	1.0	78.4
rps15-ycf1_p3	AGCAGATCCTGTGCCTTTTT	TTGATTCGCCATGCTCAAGA	45.0	45.0	57.7	57.9	1245-1269 bp	1.0	78.2
rps15-ycf1_p4	CAGCAGATCCTGTGCCTTTT	CAGCCACCGTCTAGAAGAAC	50.0	55.0	58.5	58.0	1309-1333 bp	1.0	77.7
rps15-ycf1_p5	AGCAGATCCTGTGCCTTTTT	AGTTGATTCGCCATGCTCAA	45.0	45.0	57.7	58.2	1247-1271 bp	1.0	77.7