Hotspot

ndhH-rps15

Genome · 123355-123465 · 111 bp

Back to Sphaeropteris

Pi 0.027

MarkerSeek score 68.00

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: Genome
length_bp: 111
variable_sites: 3
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GGAGAACACCATGCATCGAT	CGCAAGCGTCTCTCAATCTA	50.0	50.0	58.0	57.8	286 bp	1.0	79.0
ndhH-rps15_p2	GGAGAACACCATGCATCGAT	ATTCGAGGATTAAAGGGGCG	50.0	50.0	58.0	57.7	214 bp	1.0	78.7
ndhH-rps15_p3	CAATAAGCCGGAGAACACCA	CGCAAGCGTCTCTCAATCTA	50.0	50.0	57.9	57.8	295 bp	1.0	78.4
ndhH-rps15_p4	ACAATAAGCCGGAGAACACC	CGCAAGCGTCTCTCAATCTA	50.0	50.0	57.9	57.8	296 bp	1.0	78.4
ndhH-rps15_p5	CAATAAGCCGGAGAACACCA	ATTCGAGGATTAAAGGGGCG	50.0	50.0	57.9	57.7	223 bp	1.0	78.2