Hotspot

ndhF-rpl21

Genome · 112448-112669 · 222 bp

Back to Sphaeropteris

Pi 0.018

MarkerSeek score 62.90

Primer available yes

Label ndhF-rpl21

Feature Metadata

label_name: ndhF-rpl21
feature_type: igs
region: Genome
length_bp: 222
variable_sites: 4
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl21_p1	GCTACTAGTGGGCACAAAGG	GGTTCCACTCGGAGTTGTTT	55.0	50.0	58.3	58.0	334-337 bp	1.0	78.6
ndhF-rpl21_p2	ACTAGTGGGCACAAAGGAAC	GGTTCCACTCGGAGTTGTTT	50.0	50.0	57.7	58.0	331-334 bp	1.0	78.5
ndhF-rpl21_p3	GCTACTAGTGGGCACAAAGG	TGTTTCCCACCAACGTCAAT	55.0	45.0	58.3	57.9	319-322 bp	1.0	78.4
ndhF-rpl21_p4	ACTAGTGGGCACAAAGGAAC	TGTTTCCCACCAACGTCAAT	50.0	45.0	57.7	57.9	316-319 bp	1.0	78.4
ndhF-rpl21_p5	GCGAAGACCTTTCGTAGCTT	GGTTCCACTCGGAGTTGTTT	50.0	50.0	58.3	58.0	392-395 bp	1.0	78.2