Hotspot

petA-psbJ

Genome · 62376-63128 · 753 bp

Back to Sphaeropteris

Pi 0.0173

MarkerSeek score 68.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 753
variable_sites: 13
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAGATCTTTCTCGTG	ATAGGCTCGCTGGGGATATT	50.0	50.0	57.9	58.0	884-913 bp	1.0	80.2
petA-psbJ_p2	TCCAGTTGGCGGAAATGAAT	ATAGGCTCGCTGGGGATATT	45.0	50.0	57.8	58.0	839-868 bp	1.0	79.3
petA-psbJ_p3	GCACAGATCTTTCTCGTGCT	ATAGGCTCGCTGGGGATATT	50.0	50.0	58.3	58.0	882-911 bp	1.0	78.7
petA-psbJ_p4	CCGTTGCGTATTCAAGGTCT	ATAGGCTCGCTGGGGATATT	50.0	50.0	58.3	58.0	930-959 bp	1.0	78.6
petA-psbJ_p5	TGGCACAGATCTTTCTCGTG	TAGGCTCGCTGGGGATATTT	50.0	50.0	57.9	58.3	883-912 bp	1.0	78.4