Hotspot

accD-psaI

Genome · 57849-58413 · 565 bp

Back to Sphaeropteris

Pi 0.00708

MarkerSeek score 62.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 565
variable_sites: 4
pi: 0.00708
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAAGGGTGTTTTGAGCGAGA	AGGGTAGATAGGACGCTGTC	50.0	55.0	58.1	58.0	648-656 bp	1.0	79.4
accD-psaI_p2	GAAGGGTGTTTTGAGCGAGA	GAGGGTAGATAGGACGCTGT	50.0	55.0	58.1	58.0	649-657 bp	1.0	79.4
accD-psaI_p3	AGCCTTATTACTCAGCCCCT	AGGGTAGATAGGACGCTGTC	50.0	55.0	57.8	58.0	622-630 bp	1.0	78.8
accD-psaI_p4	AGCCTTATTACTCAGCCCCT	GAGGGTAGATAGGACGCTGT	50.0	55.0	57.8	58.0	623-631 bp	1.0	78.8
accD-psaI_p5	TGAGCGAGATATCTGAGCCT	AGGGTAGATAGGACGCTGTC	50.0	55.0	57.7	58.0	637-645 bp	1.0	78.1