Hotspot

rps4-trnL-CAA

Genome · 47327-47980 · 654 bp

Back to Sphaeropteris

Pi 0.0107

MarkerSeek score 64.90

Primer available yes

Label rps4-trnL-CAA

Feature Metadata

label_name: rps4-trnL-CAA
feature_type: igs
region: Genome
length_bp: 654
variable_sites: 7
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-CAA_p1	TCCCTGTAAGCCCTGGTAAA	CTGAATCTGGGAGCTGTCAC	50.0	55.0	58.0	58.0	812-813 bp	1.0	80.1
rps4-trnL-CAA_p2	TTCCCTGTAAGCCCTGGTAA	CTGAATCTGGGAGCTGTCAC	50.0	55.0	58.0	58.0	813-814 bp	1.0	80.1
rps4-trnL-CAA_p3	TTTCCCTGTAAGCCCTGGTA	CTGAATCTGGGAGCTGTCAC	50.0	55.0	58.0	58.0	814-815 bp	1.0	80.1
rps4-trnL-CAA_p4	AAGCAGATTGATCAGCAGCA	CTGAATCTGGGAGCTGTCAC	45.0	55.0	57.9	58.0	860-861 bp	1.0	79.5
rps4-trnL-CAA_p5	AGCAGATTGATCAGCAGCAA	CTGAATCTGGGAGCTGTCAC	45.0	55.0	57.9	58.0	859-860 bp	1.0	79.5