Hotspot

trnfM-CAU-rps14

Genome · 38261-38402 · 142 bp

Back to Sphaeropteris

Pi 0.0352

MarkerSeek score 71.00

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: Genome
length_bp: 142
variable_sites: 5
pi: 0.0352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	CGACCCAGATCCAATTACCG	50.0	55.0	58.0	58.1	287-308 bp	1.0	80.0
trnfM-CAU-rps14_p2	TTTGAACCCGTGACCTCAAG	CGACCCAGATCCAATTACCG	50.0	55.0	58.0	58.1	304-325 bp	1.0	79.9
trnfM-CAU-rps14_p3	ACAGGATTTGAACCCGTGAC	CGACCCAGATCCAATTACCG	50.0	55.0	58.1	58.1	310-331 bp	1.0	79.3
trnfM-CAU-rps14_p4	GATTTGAACCCGTGACCTCA	CGACCCAGATCCAATTACCG	50.0	55.0	57.8	58.1	306-327 bp	1.0	78.8
trnfM-CAU-rps14_p5	CCTTTGCGGAGACAGGATTT	CGACCCAGATCCAATTACCG	50.0	55.0	58.2	58.1	321-342 bp	1.0	78.7