Hotspot

matK-rps16

Genome · 3046-4026 · 981 bp

Back to Sphaeropteris

Pi 0.00719

MarkerSeek score 63.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 981
variable_sites: 7
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TTTCCGTAACGTCCCAAACC	GGGCAAAGGTACCTGAACAA	50.0	50.0	58.4	58.0	1064-1067 bp	1.0	77.8
matK-rps16_p2	ACTTTTCCGTAACGTCCCAA	GGGCAAAGGTACCTGAACAA	45.0	50.0	57.4	58.0	1067-1070 bp	1.0	76.1
matK-rps16_p3	TTTCCGTAACGTCCCAAACC	ATATCGAAACGGGCAAAGGT	50.0	45.0	58.4	57.6	1074-1077 bp	1.0	74.5
matK-rps16_p4	TTTCCGTAACGTCCCAAACC	GGGGCTCAAACAACAGAAAC	50.0	50.0	58.4	57.5	1104-1107 bp	1.0	73.7
matK-rps16_p5	TTTCCGTAACGTCCCAAACC	ATCGAAACGGGCAAAGGTAC	50.0	50.0	58.4	58.6	1072-1075 bp	1.0	73.4