Hotspot

rps3-rps19

LSC · 98846-99597 · 752 bp

Back to Speranskia

Pi 0.0623

MarkerSeek score 86.30

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 68
pi: 0.0623
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	GTCGGAAACCCAGTGGATTT	AAATTTCCGGGGACATGCAA	50.0	45.0	58.1	58.4	832-926 bp	1.0	85.6
rps3-rps19_p2	GTCGGAAACCCAGTGGATTT	AATTTCCGGGGACATGCAAA	50.0	45.0	58.1	58.4	831-925 bp	1.0	85.6
rps3-rps19_p3	GTCGGAAACCCAGTGGATTT	ATTTCCGGGGACATGCAAAA	50.0	45.0	58.1	58.4	830-924 bp	1.0	85.6
rps3-rps19_p4	GTACCAAGTCGGAAACCCAG	ATTTCCGGGGACATGCAAAA	55.0	45.0	58.2	58.4	837-931 bp	1.0	84.7
rps3-rps19_p5	GTCGGAAACCCAGTGGATTT	GTGGGCCATAAATTGGGAGA	50.0	50.0	58.1	57.5	867-961 bp	1.0	84.5