Hotspot

petA-psbJ

LSC · 75250-76506 · 1257 bp

Back to Speranskia

Pi 0.0528

MarkerSeek score 87.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1257
variable_sites: 95
pi: 0.0528
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1060-1393 bp	1.0	85.8
petA-psbJ_p2	GTTCTTCTTGGCGTCGGTTA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1052-1385 bp	1.0	85.4
petA-psbJ_p3	GCCTTTTGTTCTTCTTGGCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1059-1392 bp	1.0	85.2
petA-psbJ_p4	CTTGGCGTCGGTTATTTTGG	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.7	57.6	1046-1379 bp	1.0	85.0
petA-psbJ_p5	TTTTGTTCTTCTTGGCGTCG	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.3	57.6	1056-1389 bp	1.0	81.1