Hotspot

accD-psaI

LSC · 69456-71174 · 1719 bp

Back to Speranskia

Pi 0.0766

MarkerSeek score 85.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1719
variable_sites: 148
pi: 0.0766
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCGTTAAAAGGCGTTCTGA	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.5	58.0	1172-2918 bp	1.0	85.7
accD-psaI_p2	TCCGTTAAAAGGCGTTCTGA	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.5	58.0	1156-2902 bp	1.0	85.4
accD-psaI_p3	TCCGTTAAAAGGCGTTCTGA	AAGGCACAAAAATGGAGGGT	45.0	45.0	57.5	57.9	1132-2878 bp	1.0	85.0
accD-psaI_p4	TGAGTTACTTCAGCTCCACG	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.3	58.0	1151-2897 bp	1.0	84.2
accD-psaI_p5	TGAGTTACTTCAGCTCCACG	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.3	58.0	1135-2881 bp	1.0	83.9