Hotspot

ndhC-trnV-UAC

LSC · 59656-61891 · 2236 bp

Back to Speranskia

Pi 0.189

MarkerSeek score 79.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 2236
variable_sites: 407
pi: 0.189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACGCCCAGAATATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	37.5	50.0	57.8	57.8	817-2306 bp	1.0	56.6
ndhC-trnV-UAC_p2	AGAAACGCCCAGAATATATCA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.5	57.8	821-2310 bp	1.0	54.4
ndhC-trnV-UAC_p3	ACGCCCAGAATATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	37.5	55.0	57.8	57.5	816-2305 bp	1.0	53.9
ndhC-trnV-UAC_p4	ACGCCCAGAATATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	37.5	55.0	57.8	58.7	805-2294 bp	1.0	52.8
ndhC-trnV-UAC_p5	CGCCCAGAATATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	39.1	50.0	56.2	57.8	816-2305 bp	1.0	52.0