Hotspot

trnE-UUC-trnT-GGU

LSC · 36715-38523 · 1809 bp

Back to Speranskia

Pi 0.0753

MarkerSeek score 88.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1809
variable_sites: 192
pi: 0.0753
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1912 bp	0.333333	51.2
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1891 bp	0.333333	51.0
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	1891 bp	0.333333	50.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	1870 bp	0.333333	50.6
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	1888 bp	0.333333	50.5