Hotspot

trnG-GCC-trnR-UCU

LSC · 13011-13706 · 696 bp

Back to Speranskia

Pi 0.107

MarkerSeek score 83.20

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 104
pi: 0.107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	1378-2735 bp	1.0	89.5
trnG-GCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	55.0	50.0	58.1	58.0	1381-2738 bp	1.0	89.3
trnG-GCC-trnR-UCU_p3	TTTCGCTCACGTCTTCTAGC	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	1506-2863 bp	1.0	89.1
trnG-GCC-trnR-UCU_p4	AAGGGATCATCGGCTCCATA	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	2048 bp	0.333333	52.8
trnG-GCC-trnR-UCU_p5	TAAGGGATCATCGGCTCCAT	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	2049 bp	0.333333	52.8