Hotspot

trnS-GCU-trnG-GCC

LSC · 10284-12238 · 1955 bp

Back to Speranskia

Pi 0.209
MarkerSeek score 83.80
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1955
variable_sites
541
pi
0.209
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 AATTCCAAGTCATTGCCGGA ACGAATCACACTTTTACCACT 45.0 38.1 57.8 55.5 1703-2099 bp 0.666667 48.7
trnS-GCU-trnG-GCC_p2 ATTCCAAGTCATTGCCGGAA ACGAATCACACTTTTACCACT 45.0 38.1 57.8 55.5 1702-2098 bp 0.666667 48.7
trnS-GCU-trnG-GCC_p3 AATTCCAAGTCATTGCCGGA GAACGAATCACACTTTTACCACT 45.0 39.1 57.8 57.3 1705-2101 bp 0.666667 47.6
trnS-GCU-trnG-GCC_p4 ATTCCAAGTCATTGCCGGAA AGAACGAATCACACTTTTACCAC 45.0 39.1 57.8 57.3 1705-2101 bp 0.666667 47.6
trnS-GCU-trnG-GCC_p5 ATTCCAAGTCATTGCCGGAA GAACGAATCACACTTTTACCACT 45.0 39.1 57.8 57.3 1704-2100 bp 0.666667 47.6