Hotspot

psbI-trnS-GCU

LSC · 9951-10195 · 245 bp

Back to Speranskia

Pi 0.146

MarkerSeek score 84.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 245
variable_sites: 53
pi: 0.146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	CGAATTATCCGTACCGAGGG	50.0	55.0	57.6	57.7	308-632 bp	0.666667	72.8
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAAGAA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.6	58.6	351-675 bp	0.666667	70.4
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	57.6	57.0	288-612 bp	0.666667	67.8
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	TTATCCGTACCGAGGGTTCG	50.0	55.0	57.6	59.0	304-628 bp	0.666667	67.7
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAA	CGAAAGCGTCGGATTGCTAA	50.0	50.0	57.6	59.0	337-661 bp	0.666667	67.5