Hotspot

ycf2

IRb · 86293-93204 · 6912 bp

Back to Spatholobus

Pi 0.0122

MarkerSeek score 49.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6912
variable_sites: 84
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCCTTCCTTTCTTCAAACGGA	TCAGTAGGATTCGAACCCAGA	42.9	47.6	57.2	57.9	228 bp	1.0	66.9
ycf2_p2	TCCTTCCTTTCTTCAAACGGA	TCTCAGTAGGATTCGAACCCA	42.9	47.6	57.2	57.9	230 bp	1.0	66.9
ycf2_p3	TCCTTCCTTTCTTCAAACGGA	CCCAGATGAAGTTCTGACCA	42.9	50.0	57.2	56.8	213 bp	1.0	66.6
ycf2_p4	TCCTTCCTTTCTTCAAACGGA	ACCCAGATGAAGTTCTGACC	42.9	50.0	57.2	56.8	214 bp	1.0	66.5
ycf2_p5	TGTTGGGATCCTTCCTTTCT	TCAGTAGGATTCGAACCCAGA	45.0	47.6	56.0	57.9	236 bp	1.0	65.8