Hotspot

trnL-UAG-rpl32

SSC · 123132-123600 · 469 bp

Back to Spatholobus

Pi 0.0649
MarkerSeek score 73.40
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
469
variable_sites
30
pi
0.0649
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TGTAAAGAAGGGGTTCGGTT 50.0 45.0 57.9 56.7 1109-1114 bp 1.0 72.7
trnL-UAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA TTGTAAAGAAGGGGTTCGGT 50.0 45.0 57.9 56.7 1110-1115 bp 1.0 72.7
trnL-UAG-rpl32_p3 AGCAGCGTGTCTACCAATTT TGTAAAGAAGGGGTTCGGTT 45.0 45.0 57.8 56.7 1102-1107 bp 1.0 72.2
trnL-UAG-rpl32_p4 AGCAGCGTGTCTACCAATTT TTGTAAAGAAGGGGTTCGGT 45.0 45.0 57.8 56.7 1103-1108 bp 1.0 72.2
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TGTAAAGAAGGGGTTCGGTT 55.0 45.0 57.8 56.7 1107-1112 bp 1.0 72.1