Pi
0.0339
MarkerSeek score
70.10
Primer available
yes
Label
petA-psbL
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| petA-psbL_p1 | TGAGAAGGTTCAATTGTCCGA | CTCTACTGGGGGTTATTACTCA | 42.9 | 45.5 | 57.2 | 56.1 | 1112-1125 bp | 1.0 | 43.9 |
| petA-psbL_p2 | TGAGAAGGTTCAATTGTCCGA | TCTACTGGGGGTTATTACTCA | 42.9 | 42.9 | 57.2 | 54.8 | 1111-1124 bp | 1.0 | 43.9 |
| petA-psbL_p3 | TGAGAAGGTTCAATTGTCCGAA | CTCTACTGGGGGTTATTACTCA | 40.9 | 45.5 | 57.9 | 56.1 | 1112-1125 bp | 1.0 | 43.9 |
| petA-psbL_p4 | TTGAGAAGGTTCAATTGTCCGA | CTCTACTGGGGGTTATTACTCA | 40.9 | 45.5 | 57.9 | 56.1 | 1113-1126 bp | 1.0 | 43.9 |
| petA-psbL_p5 | GGTCTCTTGTTTTTCTTGGCA | CTCTACTGGGGGTTATTACTCA | 42.9 | 45.5 | 56.9 | 56.1 | 1180-1193 bp | 1.0 | 43.7 |