Hotspot

psaI-ycf4

LSC · 59435-59633 · 199 bp

Back to Spatholobus

Pi 0.0754

MarkerSeek score 73.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 15
pi: 0.0754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	CCCGTAGATGTTCTAATCGCA	45.0	47.6	58.0	57.6	294-298 bp	1.0	72.9
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	ACCCGTAGATGTTCTAATCGC	45.0	47.6	58.0	57.6	295-299 bp	1.0	72.9
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	CCCGTAGATGTTCTAATCGCA	45.0	47.6	57.9	57.6	288-292 bp	1.0	72.4
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	ACCCGTAGATGTTCTAATCGC	45.0	47.6	57.9	57.6	289-293 bp	1.0	72.4
psaI-ycf4_p5	GGCCTAGTATTTCCGGCAAT	CCCGTAGATGTTCTAATCGCA	50.0	47.6	57.7	57.6	302-306 bp	1.0	70.9