Hotspot

trnG-GCC-psbZ

LSC · 24066-24867 · 802 bp

Back to Spatholobus

Pi 0.0503

MarkerSeek score 78.60

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 38
pi: 0.0503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CTTGAGGTCACGGGTTCAAA	CAACCACTCAGCCATCTCTC	50.0	55.0	58.0	58.0	1565-1584 bp	1.0	85.5
trnG-GCC-psbZ_p2	CGATCATAGGAGGGTCCCTT	CAACCACTCAGCCATCTCTC	55.0	55.0	58.0	58.0	1725-1744 bp	1.0	85.1
trnG-GCC-psbZ_p3	CGTTGTTTTTCGACCGGAAG	CCTCGATCCATGCCTCAAAA	50.0	50.0	58.0	58.0	1696-1715 bp	1.0	85.1
trnG-GCC-psbZ_p4	CGTTGTTTTTCGACCGGAAG	TCGATCCATGCCTCAAAAGG	50.0	50.0	58.0	58.0	1694-1713 bp	1.0	85.1
trnG-GCC-psbZ_p5	CGTTGTTTTTCGACCGGAAG	CAACCACTCAGCCATCTCTC	50.0	55.0	58.0	58.0	1899-1917 bp	1.0	84.8