Hotspot

rps4-trnS-GGA

LSC · 15316-15534 · 219 bp

Back to Spatholobus

Pi 0.0639

MarkerSeek score 78.90

Primer available yes

Label rps4-trnS-GGA

Feature Metadata

label_name: rps4-trnS-GGA
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 14
pi: 0.0639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnS-GGA_p1	TGACTCTTCACCCATTCCAA	GTAGGAAAGATGGCCGAGTG	45.0	55.0	56.4	58.1	426-431 bp	1.0	69.4
rps4-trnS-GGA_p2	TTGACTCTTCACCCATTCCA	GTAGGAAAGATGGCCGAGTG	45.0	55.0	56.4	58.1	427-432 bp	1.0	69.4
rps4-trnS-GGA_p3	TGACTCTTCACCCATTCCAA	CCGGTTTCTGGGCATCATTA	45.0	50.0	56.4	57.9	447 bp	0.5	46.5
rps4-trnS-GGA_p4	TTGACTCTTCACCCATTCCA	CCGGTTTCTGGGCATCATTA	45.0	50.0	56.4	57.9	448 bp	0.5	46.5
rps4-trnS-GGA_p5	TTGACTCTTCACCCATTCCAA	CCGGTTTCTGGGCATCATTA	42.9	50.0	57.1	57.9	448 bp	0.5	44.6