Hotspot

trnT-UGU-rps4

LSC · 14427-14709 · 283 bp

Back to Spatholobus

Pi 0.0471
MarkerSeek score 73.10
Primer available yes
Label trnT-UGU-rps4

Feature Metadata

label_name
trnT-UGU-rps4
feature_type
igs
region
LSC
length_bp
283
variable_sites
13
pi
0.0471
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-rps4_p1 CTCAGAGGTTAGAGCATCGC AGGTCCTCTGTAACGTGACA 55.0 50.0 57.9 58.0 362-368 bp 1.0 83.3
trnT-UGU-rps4_p2 ATGGTCATCGGTTCGAATCC AGGTCCTCTGTAACGTGACA 50.0 50.0 57.8 58.0 330-336 bp 1.0 82.8
trnT-UGU-rps4_p3 CCGCTTAGCTCAGAGGTTAG AGGTCCTCTGTAACGTGACA 55.0 50.0 57.5 58.0 370-376 bp 1.0 80.2
trnT-UGU-rps4_p4 CTCAGAGGTTAGAGCATCGC AACGAGGTCCTCTGTAACGT 55.0 50.0 57.9 58.4 366-372 bp 1.0 80.2
trnT-UGU-rps4_p5 CTCAGAGGTTAGAGCATCGC AGTCCTGGTAAAGACCCCAG 55.0 55.0 57.9 58.3 403-409 bp 1.0 80.1