Hotspot

trnH-GUG-psbA

LSC · 92-384 · 293 bp

Back to Spatholobus

Pi 0.0636
MarkerSeek score 76.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
293
variable_sites
18
pi
0.0636
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCGAGCTAACCTTGGTATGG 55.0 55.0 58.1 57.8 411-412 bp 1.0 82.5
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCGAGCTAACCTTGGTATGG 50.0 55.0 57.9 57.8 425-426 bp 1.0 82.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCGAGCTAACCTTGGTATGG 50.0 55.0 57.9 57.8 424-425 bp 1.0 82.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCGAGCTAACCTTGGTATGG 50.0 55.0 57.9 57.8 426-427 bp 1.0 82.2
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GATTTAGCTGCGATCGAAGC 55.0 50.0 58.1 57.4 353-354 bp 1.0 80.8