Hotspot

tRNA-Asp(GUC)

LSC · 33412-33483 · 72 bp

Back to Spathiphyllum

Pi 0.0417

MarkerSeek score 74.10

Primer available yes

Label tRNA-Asp(GUC)

Feature Metadata

label_name: tRNA-Asp(GUC)
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 3
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asp(GUC)_p1	CCCAAGCAGTAGAAGTGCAA	ATGTATGCTGTACATCCCGC	50.0	50.0	58.1	57.8	192-794 bp	1.0	78.3
tRNA-Asp(GUC)_p2	TGGCTATAGACCCAAGCAGT	ATGTATGCTGTACATCCCGC	50.0	50.0	58.1	57.8	202-804 bp	1.0	78.2
tRNA-Asp(GUC)_p3	GACCCAAGCAGTAGAAGTGC	ATGTATGCTGTACATCCCGC	55.0	50.0	58.6	57.8	194-796 bp	1.0	74.7
tRNA-Asp(GUC)_p4	CCCAAGCAGTAGAAGTGCAA	TGTATGCTGTACATCCCGCT	50.0	50.0	58.1	58.9	191-793 bp	1.0	72.6
tRNA-Asp(GUC)_p5	TGGCTATAGACCCAAGCAGT	TGTATGCTGTACATCCCGCT	50.0	50.0	58.1	58.9	201-803 bp	1.0	72.5