Hotspot

rps15-ycf1

SSC · 131159-132127 · 969 bp

Back to Spathiphyllum

Pi 0.169

MarkerSeek score 83.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 969
variable_sites: 151
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTCGTAAACCCCTCCGAGAT	TTGGCGGGGTAATGTTGTAG	50.0	50.0	58.1	57.9	1424-1452 bp	1.0	89.9
rps15-ycf1_p2	ATCAGTCGAAGAACACCGTG	CTTGGCGGGGTAATGTTGTA	50.0	50.0	57.9	57.9	1737-1760 bp	1.0	89.6
rps15-ycf1_p3	ATCAGTCGAAGAACACCGTG	TTGGCGGGGTAATGTTGTAG	50.0	50.0	57.9	57.9	1736-1759 bp	1.0	89.6
rps15-ycf1_p4	TTCCCAATTGTTCTGGTGCA	CTTGGCGGGGTAATGTTGTA	45.0	50.0	57.9	57.9	1922-1945 bp	1.0	88.4
rps15-ycf1_p5	TTCCCAATTGTTCTGGTGCA	TTGGCGGGGTAATGTTGTAG	45.0	50.0	57.9	57.9	1921-1944 bp	1.0	88.4