Hotspot

ndhE-ndhG

SSC · 125432-125819 · 388 bp

Back to Spathiphyllum

Pi 0.0503

MarkerSeek score 83.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 388
variable_sites: 16
pi: 0.0503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GTGCTCTAACCATGCTTCGA	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	518-519 bp	1.0	81.7
ndhE-ndhG_p2	TGCTCTAACCATGCTTCGAC	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	517-518 bp	1.0	81.7
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	519-520 bp	1.0	81.7
ndhE-ndhG_p4	AACCATGCTTCGACTTGTGA	AGTTGCCTTGATAGGTGCAA	45.0	45.0	57.7	57.4	511-512 bp	1.0	79.8
ndhE-ndhG_p5	GCTCTAACCATGCTTCGACT	AGTTGCCTTGATAGGTGCAA	50.0	45.0	57.7	57.4	516-517 bp	1.0	79.4