Hotspot

psaC-ndhE

SSC · 124181-125125 · 945 bp

Back to Spathiphyllum

Pi 0.0749

MarkerSeek score 75.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 945
variable_sites: 56
pi: 0.0749
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACTGCGAGTTGTCTCATGC	GTCGAAGCATGGTTAGAGCA	50.0	50.0	58.0	58.0	1228-1410 bp	1.0	87.5
psaC-ndhE_p2	TACTGCGAGTTGTCTCATGC	TCGAAGCATGGTTAGAGCAC	50.0	50.0	58.0	58.0	1227-1409 bp	1.0	87.5
psaC-ndhE_p3	TACTGCGAGTTGTCTCATGC	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	1226-1408 bp	1.0	87.4
psaC-ndhE_p4	GGACAAGCGGATTCACATCT	GTCGAAGCATGGTTAGAGCA	50.0	50.0	58.0	58.0	1175-1357 bp	1.0	87.4
psaC-ndhE_p5	GGACAAGCGGATTCACATCT	TCGAAGCATGGTTAGAGCAC	50.0	50.0	58.0	58.0	1174-1356 bp	1.0	87.4